Zeiss Lattice Lightsheet


Laser Lines: 488nm, 561nm, 640nm

Suggested Software:

  • Zen 3.6 (blue edition)

The Zeiss LLS7 saves images in Zeiss’s .czi format. Post processing is required (deconvolution and deskewing) in order to reach the claimed resolution and produce a usable file for subsequent analysis. Arivis Vision4D is also available for iMAP users.

Additional Information:

  • Equipped for live imaging, has gas
  • Simultaneous data processing workstation: IntelR XeonR Gold, 16 cores, 128GB RAM, NVIDIA RTXA4000 48GB VRAM

 
Metadata Instrument Identifier: imap-zeiss-lls7
If you are annotating your data in preparation for data deposit or format conversion (to Neurodata without borders or other file type for long term preservation) please include the above identifier to indicate the data was taken with this instrument.

Serial Number: 2586000108

Citation Guidelines
To describe the iMAP Zeiss Lattice Light Sheet 7 in your paper’s methods section please modify this example:

“Images were collected on a Zeiss Lattice Light Sheet 7. Fluorophores were excited with [list wavelengths here] with exposure times ranging from [list exposure time range here].”

 
Wavelengths of excitation and wavelength ranges for emission are in the metadata of the .czi files from the Zeiss LLS7. Please contact Jeffrey LeDue (jledue@mail.ubc.ca) if you need help finding this information and tailoring the above statement.