Deep Multiphoton


Objectives:

Name Magnification Medium Numerical Aperture Working Distance (mm) Additional Features
XLFLUOR2X/340 2x Air (Water:0-5mm) 0.14 19.8 – 21.0
CFI Plan Apochromat Lambda D 10X 10x Air 0.45 4
Olympus XLUMPlan FL 20x Water 0.95 0.17
Olympus XLPLN25XWMP2 25x Water 1.05 2 Correction Collar – Brain sample index mismatch
Cover Glass: 0 – 0.23mm

Excitation Source: Coherent Chameleon S.N. GDP.1001H.2051

Filters: Blue, Green, Red

Detectors: Hamamatsu type H16201P-40-02

Suggested Software: Scanimage

Metadata Instrument Identifier: imap-3p
If you are annotating your data in preparation for data deposit or format conversion (to Neurodata without borders or other file type for long term preservation) please include the above identifier to indicate the data was taken with this instrument.

Citation Guidelines
To describe the iMAP 3p system in your paper’s methods section please modify this example:

“Images were recorded by [single (470nm or 565nm), 2-photon (e.g. 920nm), and/or 3-photon (1300nm)] fluorescence excitation using a custom microscope provided by COSYS Ltd East Sussex, UK”

Please contact Jeffrey LeDue (jledue@mail.ubc.ca) if you need help finding this information and tailoring the above statement.