Zeiss Live Cell Imaging System

Metadata Instrument Identifier: ninc-zeiss-live

If you are annotating your data in preparation for data deposit or format conversion (to Neurodata without borders or other file type for long term preservation) please include the above identifier to indicate the data was taken with this instrument.

Paper Acknowledgement Section Suggested text:

If you are publishing your work and have used NINC resources in your project please use the following text including the persistent identifier for NINC (Research Resource ID, RRID):

This work was supported by resources made available through the Dynamic Brain Circuits cluster and the NeuroImaging and NeuroComputation Centre at the UBC Djavad Mowafaghian Centre for Brain Health (RRID SCR_019086).”

Paper Methods Section Example Microscope Description:

To describe the NINC Zeiss Live Cell Imaging System in your paper’s methods section please modify this example: “Images were collected on a NINC Zeiss Live Cell Imaging System equipped with a LED based Colibri excitation source. Fluorophores were excited with <list wavelengths, 365nm, 473nm, 589nm > and emission was collected in these <list wavelength ranges> wavelength ranges with a <5, 10, 25, 40, and/or 63X> objective lens.”

Wavelengths of excitation and wavelength ranges for emission are in the metadata of the .czi files from the Zeiss scope. Please contact Jeffrey LeDue (jledue@mail.ubc.ca) if you need help finding this information and tailoring the above statement.

Suggested Software:

The NINC Zeiss Live Cell Imaging System saves images in Zeiss’s .czi format. The .czi format can be read directly with ImageJ. Please download and install the Fiji versions of imagej. Fiji uses the Bio-Formats importer to work directly with .czi files.

Access and Training:

Access to the NINC Zeiss Live Cell Imaging System is restricted to trained users from NINC member labs. If your lab needs NINC membership please contact Daphne Druick in DMCBH Administration (ddruick@mail.ubc.ca) and cc Jeffrey LeDue (jledue@mail.ubc.ca) to arrange payment of membership fees. Training sessions are included in the NINC membership fee. Please contact Jeffrey LeDue (jledue@mail.ubc.ca) to arrange a training session.