Multiphoton Mesoscope

Metadata Instrument Identifier: imap-meso2p

If you are annotating your data in preparation for data deposit or format conversion (to Neurodata without borders or other file type for long term preservation) please include the above identifier to indicate the data was taken with this instrument.

Paper Acknowledgement Section Suggested text:

If you are publishing your work and have used NINC resources in your project please use the following text including the persistent identifier for NINC (Research Resource ID, RRID):

This work was supported by resources made available through the Dynamic Brain Circuits cluster and the NeuroImaging and NeuroComputation Centre at the UBC Djavad Mowafaghian Centre for Brain Health (RRID SCR_019086).”

Paper Methods Section Example Instrument Description:

To describe the iMAP Multiphoton Mesoscope system in your paper’s methods section please modify this example: “Two photon images were recorded using a multiphoton mesoscope (COSYS Ltd, East Sussex, UK) based on the diesel2p <ref1>. Fluorescence was excited using a Ti:Sapphire laser at 920nm.”

<ref1> https://pubmed.ncbi.nlm.nih.gov/34789723/


Please contact Jeffrey LeDue (jledue@mail.ubc.ca) if you need help finding this information and tailoring the above statement.

Suggested Software:

Two photon calcium imaging data processing is usually performed with open source packages such as CaImAn or suite2p. Code is available and we have assembled a guide on UBC oneDrive. Please contact jledue@mail.ubc.ca for access. Additional support is available through the Brain Circuits cluster Databinge meeting.

Access and Training:

Access to iMAP equipment is restricted to trained users from the iMAP Project team. Due to the complexity and expense of iMAP equipment, this project has its own fee structure and oversight committee. If your lab is interested in joining the iMAP Project please contact Jeffrey LeDue (jledue@mail.ubc.ca) initiate a request to the oversight committee.