Lattice Lightsheet Microscopy

Metadata Instrument Identifier: imap-zeiss-lls7

If you are annotating your data in preparation for data deposit or format conversion (to Neurodata without borders or other file type for long term preservation) please include the above identifier to indicate the data was taken with this instrument.

Paper Acknowledgement Section Suggested text:

If you are publishing your work and have used NINC resources in your project please use the following text including the persistent identifier for NINC (Research Resource ID, RRID):

This work was supported by resources made available through the Dynamic Brain Circuits cluster and the NeuroImaging and NeuroComputation Centre at the UBC Djavad Mowafaghian Centre for Brain Health (RRID SCR_019086).”

Paper Methods Section Example Microscope Description:

To describe the iMAP Zeiss Lattice Light Sheet 7 in your paper’s methods section please modify this example: “Images were collected on a Zeiss Lattice Light Sheet 7. Fluorophores were excited with <list wavelengths> with exposure times ranging from <list exposure time range>.”

Wavelengths of excitation and wavelength ranges for emission are in the metadata of the .czi files from the Zeiss LLS7. Please contact Jeffrey LeDue (jledue@mail.ubc.ca) if you need help finding this information and tailoring the above statement.

Suggested Software:

The Zeiss LLS7 saves images in Zeiss’s .czi format. Post processing is required (deconvolution and deskewing) in order to reach the claimed resolution and produce a usable file for subsequent analysis. Arivis Vision4D is also available for iMAP users.

Access and Training:

Access to the iMAP Zeiss LLS7 is restricted to trained users from iMAP labs. If your lab needs access please contact Jeffrey LeDue (jledue@mail.ubc.ca) to initiate a request to the iMAP oversight committee.